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KMID : 0545120040140040836
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 4 p.836 ~ p.843
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Transcriptional Responses of Human Respiratory Epithelial Cells to Nontypeable Haemophilus influenzae Infection Analyzed by High Density cDNA Microarrays
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Lee JY
Lee NG
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Abstract
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Nontypeable H. influenzae (NTHi) a Gram-negative obligate human pathogen causes pneumonia chronic bronchitis and otitis media and the respiratory epithelium is the first line of defense that copes with the pathogen. In an effort to identify transcriptional responses of human respiratory epithelial cells to infection with NTHi we examined its differential gene expression using high density cDNA microarrays. BEAS-2B human bronchial epithelial cells were exposed to NTHi for 3 h and 24 h and the alteration of mRNA expression was analyzed using microarrays consisting of 8170 human cDNA clones. The results indicated that approximately 2.6% of the genes present on the microarrays increased in expression over 2-fold and 3.8% of the genes decreased during the 24-h infection period. Upregulated genes included cytokines (granulocyte-macrophage colony stimulating factor 2 granulocyte chemotactic protein 2 IL-6 IL-10 IL-8) transcription factors (Kruppel-like factor 7 CCAAT/enhancer binding protein β E2F-1 NF-κB) cell surface molecules (CD74 ICAM-1 ICAM-2 HLA class I) as well as those involved in signal transduction and cellular transport. Selected genes were further confirmed by reverse-transcription-PCR. These data expand our knowledge of host cellular responses during NTHi infection and should provide a molecular basis for the study of host-NTHi interaction.
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